280001-2
Boekel Scientific Programmable Slide Moat, 280001, (115V/230V)The Boekel Scientific Programmable Slide Moat helps your team go FISH-ing for better reactions. Designed for use with all fluorescent in situ hybridization protocols, the digital microprocessor control offers your team complete programmability, precise temperature control and a sealed chamber in a compact footprint.
This Boekel Programmable Slide Moat includes 12-slide capacity, a friction hinged lid, a calibration mode and four leveling feet to accommodate any bench. The Boekel Scientific programmable slide hybridization system offers researchers a compact 12 position Slide Hybridization system capable of performing complex IHC and InSitu Hybridization reactions. The digital microprocessor control offers full programmability of up to three time controlled temperature set points. The precision temperature control and sealed chamber offer the ideal environment for controlled IHC and InSitu Hybridization reactions. The Boekel Scientific Programmable Slide Hybridization system offers greater functionality with a compact footprint that compliments our complete line of Hybridization and Slide Hybridization systems.
Applications
FISH OverviewMolecular diagnostics is the fastest growing segment of the diagnostics market. Flourescent In Situ Hybridization or FISH analysis comprises 700 Million of this 5 Billion dollar market. The FISH market is projected to grow at 11% year over year for the next five years. 400 to 500 million of this market is generated in the United States. FISH testing is used to identify biological markers in the form of DNA/ RNA. It is well-known method for genetic mapping and gene expression profiling. This technique uses fluorescent dye-labeled probes and a nucleotide sequence that is mapped to a chromosome or intra-nuclear DNA to generate a fluorescence signal which is visualized in situ by microscopy. It is performed on fresh preparations such as cytospins(cell suspensions) and touch preps or formalin-fixed, paraffin-embedded (FFPE) tissues may be used. It is used for research and clinical diagnostics in the areas of: • Prenatal diagnosis • Cancer diagnosis • Molecular cytogenetic of birth defects and mental retardation • Chromosomal abnormalities • Clinical genetics • Neuroscience • Reproductive medicine • Toxicology • Microbial ecology • Evolutionary biology • Comparative genomics • Cellular genomics • Differentiation between benign and malignant tumors The majority of this market is for reagents including FDA approved tests, companion diagnostics, research use only probes, custom probes and support reagents and supplies. There is a need for hybridization equipment to perform these labor intensive tests. To understand the need for hybridization equipment the process requirements must clearly be defined. The equipment necessary is as follows: - pH meter - Balance - Stir plate - Micro-centrifuge - Refrigerator - Freezer Tissue preparation will require: - Microtome - Water bath - Oven Performing the FISH procedure will require the following equipment and materials in addition to reagents: - Slide dryer - Water baths - Hybridization Oven - Coplin jars - Coverslips - Staining dishes - Rubber cement (Optional: depends on equipment used) - Fluorescent microscopes DAPI filter (blue), FITC filter (green), and a Texas red filter capable of photography
The FISH Process - Simplified Sample prep – depariffinization, dehydrate, air dry, pretreatment(95C @ 30 min, Coplin Jar), protease treatment(37°C 10 – 20 min, Coplin jar), Dehydrate, Air Dry, Mark, apply fish probe, Place Cover glass with cement Denature - 5 minutes @ 75°C (45 – 50°C for metaphase spread + additional step to denature probe) Hybridization - 37°C 16hr – 72hr Labeling Process Direct Labeling – label is bound to the probe and it is less sensitive - Or - Indirect Labeling – Additional step before detection, probe detected using antibodies, results in amplified single Storage – 4 – 8°C for up to two months, in dark area – light sensitive
Crucial Process Steps Two steps in the FISH process require equipment for heating the slides; the denaturing step and the hybridization. These steps are similar for most FFPE FISH procedures. Denature: Denaturing the probes and double stranded DNA Average Time: 5 Minutes Standard Temperature: 75°C Hybridization: Hybridization of the probes to the target nucleic acids Time Range: 16 – 72 Hours Standard Temperature: 37°C Developed slides must be stored at 4 – 8°C until they are evaluated under a microscope and photographed. They can be store for months at these temperatures.
List of Specific FISH Tests There are thousands of FISH probes used for research supplied by dozens of companies. These do not have FDA approval. The FDA approved test that use the FISH procedure are listed below.
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