18, Jalan Sungai Burung U32/U,
Seksyen 32, Bukit Rimau,
40460 Shah Alam,
Selangor Darul Ehsan, Malaysia.
![]() Boekel Scientific RapidFISH Slide Hybridizer, 240200, Microscope Slide Oven (115V/230V)The RapidFISH was designed to simplify Fluorescent In-Situ Hybridization (FISH) procedures. The unit has a self-clamping slide tray design that maintains the required humidity. The control system provides excellent temperature stability and uniformity. Small benchtop design saves space in your lab. The RapidFISH was designed to simplify Fluorescent In-Situ Hybridization (FISH) procedures for FFPE (formalin fixed paraffin embedded tissue) samples, cell suspensions, and other tissue preparation procedures. Temperature control and humidity are essential for FISH probes to bind to RNA and DNA. The RapidFISH has a self-clamping slide tray design that maintains the required humidity. Microscope slides in the RapidFISH do not require coverslips to be glued because the removable tray of the slide incubator traps moisture in the system. Elimination of this step decreases processing time and ensures that the specimens stay hydrated during processing. The system has an easy to use control system for controlling temperature, time and controlled temperature steps. The device has a small footprint and still maintains excellent temperature stability and uniformity because of its dual heater convection system. FISH testing is used worldwide to identify biological markers in the form of DNA/RNA. | ![]() Boekel Scientific Big S.H.O.T III™ Large Hybridization Oven, 230402 (115V/230V)Whether you are working with Northern Blots, Southern Blots, Microarrays or another application that requires incubation, you will find the Big SHOT III™ Hybridization Oven is a perfect fit for the job. This unit is designed to provide rapid heat-up, excellent temperature stability and efficient mixing for all types of hybridization and incubation applications. Boekel Scientific provides a full line of accessories which make it possible to accommodate many different vessel sizes, types, quantities and orientations. The oven can be converted to a platform rocker with an optional rocker platform attachment.
The Boekel Big S.H.O.T. III™ - a high capacity oven
| ![]() Boekel Scientific Slide Moat™ Slide Hybridizer, 240000, (115V/230V)Boekel Scientific Slide Moat™ Slide Hybridizer, 240000, (115V/230V)IN STOCK
SKU 240000 & 240000-2
The Slide Moat™ Slide Hybridizer from Boekel Scientific is a winner for FISH and other lab hybridization procedures. With a capacity for 30 standard microscopic slides and excellent temperature stability and uniformity, this high-quality slide moat positions your team for success. The tempered glass lid seals with an integral gasket on the heating base, and an optional light shield is available for photosensitive reagents.
Applications
FISH OverviewMolecular diagnostics is the fastest growing segment of the diagnostics market. Flourescent In Situ Hybridization or FISH analysis comprises 700 Million of this 5 Billion dollar market. The FISH market is projected to grow at 11% year over year for the next five years. 400 to 500 million of this market is generated in the United States. FISH testing is used to identify biological markers in the form of DNA/ RNA. It is well-known method for genetic mapping and gene expression profiling. This technique uses fluorescent dye-labeled probes and a nucleotide sequence that is mapped to a chromosome or intra-nuclear DNA to generate a fluorescence signal which is visualized in situ by microscopy. It is performed on fresh preparations such as cytospins(cell suspensions) and touch preps or formalin-fixed, paraffin-embedded (FFPE) tissues may be used. It is used for research and clinical diagnostics in the areas of: • Prenatal diagnosis • Cancer diagnosis • Molecular cytogenetic of birth defects and mental retardation • Chromosomal abnormalities • Clinical genetics • Neuroscience • Reproductive medicine • Toxicology • Microbial ecology • Evolutionary biology • Comparative genomics • Cellular genomics • Differentiation between benign and malignant tumors The majority of this market is for reagents including FDA approved tests, companion diagnostics, research use only probes, custom probes and support reagents and supplies. There is a need for hybridization equipment to perform these labor intensive tests. To understand the need for hybridization equipment the process requirements must clearly be defined. The equipment necessary is as follows: - pH meter - Balance - Stir plate - Micro-centrifuge - Refrigerator - Freezer Tissue preparation will require: - Microtome - Water bath - Oven Performing the FISH procedure will require the following equipment and materials in addition to reagents: - Slide dryer - Water baths - Hybridization Oven - Coplin jars - Coverslips - Staining dishes - Rubber cement (Optional: depends on equipment used) - Fluorescent microscopes DAPI filter (blue), FITC filter (green), and a Texas red filter capable of photography
The FISH Process - Simplified Sample prep – depariffinization, dehydrate, air dry, pretreatment(95C @ 30 min, Coplin Jar), protease treatment(37°C 10 – 20 min, Coplin jar), Dehydrate, Air Dry, Mark, apply fish probe, Place Cover glass with cement Denature - 5 minutes @ 75°C (45 – 50°C for metaphase spread + additional step to denature probe) Hybridization - 37°C 16hr – 72hr Labeling Process Direct Labeling – label is bound to the probe and it is less sensitive - Or - Indirect Labeling – Additional step before detection, probe detected using antibodies, results in amplified single Storage – 4 – 8°C for up to two months, in dark area – light sensitive
Crucial Process Steps Two steps in the FISH process require equipment for heating the slides; the denaturing step and the hybridization. These steps are similar for most FFPE FISH procedures. Denature: Denaturing the probes and double stranded DNA Average Time: 5 Minutes Standard Temperature: 75°C Hybridization: Hybridization of the probes to the target nucleic acids Time Range: 16 – 72 Hours Standard Temperature: 37°C Developed slides must be stored at 4 – 8°C until they are evaluated under a microscope and photographed. They can be store for months at these temperatures.
List of Specific FISH Tests There are thousands of FISH probes used for research supplied by dozens of companies. These do not have FDA approval. The FDA approved test that use the FISH procedure are listed below.
| ![]() Boekel Scientific Bambino II™ Hybridization Oven, 230301 (115V/230V)The Bambino II Hybridization Oven Model 230301 from Boekel Scientific saves bench space while heating up your team's microarray game. This mini hybridization oven delivers rapid heat-up, excellent temperature stability and efficient mixing for Northern Blots, Southern Blots and all types of hybridization and incubation applications in a small footprint.
Each unit comes with a carousel that holds four 50-milliliter conical tubes. Other carousels and accessories are available for separate purchase. Whether you are working with Northern Blots, Southern Blots, Microarrays or another application that requires incubation, you will find the Bambino™ Hybridization Oven is a perfect fit for the job. This unit is designed to provide rapid heat-up, excellent temperature stability and efficient mixing for all types of hybridization and incubation applications. Boekel Scientific provides a full line of accessories for both the Bambino™ Hybridization Oven which make it possible to accommodate many different vessel sizes, types, quantities and orientations.
The Boekel Big S.H.O.T. III™ - a high capacity oven
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![]() Boekel Scientific Programmable Slide Moat, 280001, (115V/230V)The Boekel Scientific Programmable Slide Moat helps your team go FISH-ing for better reactions. Designed for use with all fluorescent in situ hybridization protocols, the digital microprocessor control offers your team complete programmability, precise temperature control and a sealed chamber in a compact footprint.
This Boekel Programmable Slide Moat includes 12-slide capacity, a friction hinged lid, a calibration mode and four leveling feet to accommodate any bench. The Boekel Scientific programmable slide hybridization system offers researchers a compact 12 position Slide Hybridization system capable of performing complex IHC and InSitu Hybridization reactions. The digital microprocessor control offers full programmability of up to three time controlled temperature set points. The precision temperature control and sealed chamber offer the ideal environment for controlled IHC and InSitu Hybridization reactions. The Boekel Scientific Programmable Slide Hybridization system offers greater functionality with a compact footprint that compliments our complete line of Hybridization and Slide Hybridization systems.
Applications
FISH OverviewMolecular diagnostics is the fastest growing segment of the diagnostics market. Flourescent In Situ Hybridization or FISH analysis comprises 700 Million of this 5 Billion dollar market. The FISH market is projected to grow at 11% year over year for the next five years. 400 to 500 million of this market is generated in the United States. FISH testing is used to identify biological markers in the form of DNA/ RNA. It is well-known method for genetic mapping and gene expression profiling. This technique uses fluorescent dye-labeled probes and a nucleotide sequence that is mapped to a chromosome or intra-nuclear DNA to generate a fluorescence signal which is visualized in situ by microscopy. It is performed on fresh preparations such as cytospins(cell suspensions) and touch preps or formalin-fixed, paraffin-embedded (FFPE) tissues may be used. It is used for research and clinical diagnostics in the areas of: • Prenatal diagnosis • Cancer diagnosis • Molecular cytogenetic of birth defects and mental retardation • Chromosomal abnormalities • Clinical genetics • Neuroscience • Reproductive medicine • Toxicology • Microbial ecology • Evolutionary biology • Comparative genomics • Cellular genomics • Differentiation between benign and malignant tumors The majority of this market is for reagents including FDA approved tests, companion diagnostics, research use only probes, custom probes and support reagents and supplies. There is a need for hybridization equipment to perform these labor intensive tests. To understand the need for hybridization equipment the process requirements must clearly be defined. The equipment necessary is as follows: - pH meter - Balance - Stir plate - Micro-centrifuge - Refrigerator - Freezer Tissue preparation will require: - Microtome - Water bath - Oven Performing the FISH procedure will require the following equipment and materials in addition to reagents: - Slide dryer - Water baths - Hybridization Oven - Coplin jars - Coverslips - Staining dishes - Rubber cement (Optional: depends on equipment used) - Fluorescent microscopes DAPI filter (blue), FITC filter (green), and a Texas red filter capable of photography
The FISH Process - Simplified Sample prep – depariffinization, dehydrate, air dry, pretreatment(95C @ 30 min, Coplin Jar), protease treatment(37°C 10 – 20 min, Coplin jar), Dehydrate, Air Dry, Mark, apply fish probe, Place Cover glass with cement Denature - 5 minutes @ 75°C (45 – 50°C for metaphase spread + additional step to denature probe) Hybridization - 37°C 16hr – 72hr Labeling Process Direct Labeling – label is bound to the probe and it is less sensitive - Or - Indirect Labeling – Additional step before detection, probe detected using antibodies, results in amplified single Storage – 4 – 8°C for up to two months, in dark area – light sensitive
Crucial Process Steps Two steps in the FISH process require equipment for heating the slides; the denaturing step and the hybridization. These steps are similar for most FFPE FISH procedures. Denature: Denaturing the probes and double stranded DNA Average Time: 5 Minutes Standard Temperature: 75°C Hybridization: Hybridization of the probes to the target nucleic acids Time Range: 16 – 72 Hours Standard Temperature: 37°C Developed slides must be stored at 4 – 8°C until they are evaluated under a microscope and photographed. They can be store for months at these temperatures.
List of Specific FISH Tests There are thousands of FISH probes used for research supplied by dozens of companies. These do not have FDA approval. The FDA approved test that use the FISH procedure are listed below.
![]() | ![]() Boekel Scientific UV Crosslinker AH (115V/230V), 234100Whether you are crosslinking DNA or RNA, conducting Northern, Southern dot or slot blot analysis or UV curing, The Boekel Scientific UV Crosslinker AH provides a safe, time-efficient solution with a controlled amount of ultraviolet radiation. The researcher now gets more accurate results by utilizing our unique-to-the-market, adjustable height feature. This feature gives much more true-to-life readings by keeping samples on the base of the chamber, at the same distance from the bulbs as the fixed UV sensor. Instead of building up samples to get them closer to the bulbs simply adjust the bulbs to get them closer to your sample! Our microprocessor detects the exact energy of the bulbs and compensates accordingly.
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